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dc.contributor.authorMulroney, K.
dc.contributor.authorHall, J.
dc.contributor.authorHuang, X.
dc.contributor.authorTurnbull, E.
dc.contributor.authorBzdyl, N.
dc.contributor.authorChakera, Aron
dc.contributor.authorNaseer, U.
dc.contributor.authorCorea, E.
dc.contributor.authorEllington, M.
dc.contributor.authorHopkins, K.
dc.contributor.authorWester, A.
dc.contributor.authorEkelund, O.
dc.contributor.authorWoodford, N.
dc.contributor.authorInglis, T.
dc.date.accessioned2018-02-06T06:16:31Z
dc.date.available2018-02-06T06:16:31Z
dc.date.created2018-02-06T05:50:01Z
dc.date.issued2017
dc.identifier.citationMulroney, K. and Hall, J. and Huang, X. and Turnbull, E. and Bzdyl, N. and Chakera, A. and Naseer, U. et al. 2017. Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia. Scientific Reports. 7 (1).
dc.identifier.urihttp://hdl.handle.net/20.500.11937/63339
dc.identifier.doi10.1038/s41598-017-02009-3
dc.description.abstract

© 2017 The Author(s). The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MIC FAST ) correspond closely with broth microdilution MIC (MIC BMD , Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.

dc.publisherNature Publishing Group
dc.titleRapid susceptibility profiling of carbapenem-resistant Klebsiella pneumonia
dc.typeJournal Article
dcterms.source.volume7
dcterms.source.number1
dcterms.source.issn2045-2322
dcterms.source.titleScientific Reports
curtin.departmentCurtin Medical School
curtin.accessStatusOpen access via publisher


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