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    Quantification of cell-substratum interactions by atomic force microscopy

    Access Status
    Fulltext not available
    Authors
    Li, Q.
    Becker, Thomas
    Sand, W.
    Date
    2017
    Type
    Journal Article
    
    Metadata
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    Citation
    Li, Q. and Becker, T. and Sand, W. 2017. Quantification of cell-substratum interactions by atomic force microscopy. Colloids and Surfaces B: Biointerfaces. 159: pp. 639-643.
    Source Title
    Colloids and Surfaces B: Biointerfaces
    DOI
    10.1016/j.colsurfb.2017.08.023
    ISSN
    0927-7765
    School
    School of Molecular and Life Sciences (MLS)
    URI
    http://hdl.handle.net/20.500.11937/68139
    Collection
    • Curtin Research Publications
    Abstract

    Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2.6 ± 0.3 nN and 77.3 ± 7.1 pN, respectively. This was achieved under natural conditions without any artefact resulting from the use of denaturing chemicals such as glutaraldehyde. This new technique is unique for quantifying the real interaction forces between cells or biofilms and their substrata.

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