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dc.contributor.authorLi, Q.
dc.contributor.authorBecker, Thomas
dc.contributor.authorSand, W.
dc.date.accessioned2018-05-18T08:00:59Z
dc.date.available2018-05-18T08:00:59Z
dc.date.created2018-05-18T00:23:22Z
dc.date.issued2017
dc.identifier.citationLi, Q. and Becker, T. and Sand, W. 2017. Quantification of cell-substratum interactions by atomic force microscopy. Colloids and Surfaces B: Biointerfaces. 159: pp. 639-643.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/68139
dc.identifier.doi10.1016/j.colsurfb.2017.08.023
dc.description.abstract

Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2.6 ± 0.3 nN and 77.3 ± 7.1 pN, respectively. This was achieved under natural conditions without any artefact resulting from the use of denaturing chemicals such as glutaraldehyde. This new technique is unique for quantifying the real interaction forces between cells or biofilms and their substrata.

dc.publisherElsevier BV
dc.titleQuantification of cell-substratum interactions by atomic force microscopy
dc.typeJournal Article
dcterms.source.volume159
dcterms.source.startPage639
dcterms.source.endPage643
dcterms.source.issn0927-7765
dcterms.source.titleColloids and Surfaces B: Biointerfaces
curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusFulltext not available


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