Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Pulsed field gel electrophoresis reveals chromosome length differences between strains of Cladosporium fulvum (syn. Fulvia fulva)

    Access Status
    Fulltext not available
    Authors
    Talbot, N.
    Coddington, A.
    Oliver, Richard
    Date
    1991
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    TALBOT N, CODDINGTON A & OLIVER RP (1991) Pulsed field gel electrophoresis reveals chromosome length differences between isolates of Cladosporium fulvum (syn. Fulvia fulva). Molecular and General Genetics 229 267-272
    DOI
    10.1007/BF00272165
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/7727
    Collection
    • Curtin Research Publications
    Abstract

    Methods are described for the electrophoretic separation of chromosome-sized DNA molecules from the fungal tomato pathogen Cladosporium fulvum (syn. Fulvia fulva). Using a hexagonal electrode array and switching times of 75 min at 45 V for 14 days, nine bands could be resolved. By comparison with co-electrophoresed Aspergillus nidulans chromosomal DNA (which was resolved into seven bands), the sizes of the C. fulvum bands are estimated to be between 1.9 Mb and 5.4 Mb. The two largest bands are believed to be doublets, giving a minimum genome size of 44 Mb. Cloned probes for the ribosomal DNA repeat, an anonymous single copy fragment and a newly discovered retrotransposon were hybridized to blots of the pulsed field gels, demonstrating the use of this technique for genomic mapping. Most strains of C. fulvum had an identical pattern of bands. Two strains exhibited two polymorphisms which could be due to a translocation.

    Related items

    Showing items related by title, author, creator and subject.

    • Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum
      Arnau, J.; Oliver, Richard (1993)
      Protoplasts of a pAN7-1-transformed isolated of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector ...
    • The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum
      Arnau, J.; Oliver, Richard (1994)
      RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree ...
    • Reducing the dimensionality of hyperspectral remotely sensed data with applications for maximum likelihood image classification
      Santich, Norman Ty (2007)
      As well as the many benefits associated with the evolution of multispectral sensors into hyperspectral sensors there is also a considerable increase in storage space and the computational load to process the data. ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.