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dc.contributor.authorVan Der Heyde, Mieke
dc.contributor.authorBunce, Michael
dc.contributor.authorWardell-Johnson, Grant
dc.contributor.authorFernandes, Kristen
dc.contributor.authorWhite, Nicole
dc.contributor.authorNevill, Paul
dc.date.accessioned2022-01-24T07:29:17Z
dc.date.available2022-01-24T07:29:17Z
dc.date.issued2020
dc.identifier.citationvan der Heyde, M. and Bunce, M. and Wardell-Johnson, G. and Fernandes, K. and White, N.E. and Nevill, P. 2020. Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding. Molecular Ecology Resources. 20 (3): pp. 732-745.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/87446
dc.identifier.doi10.1111/1755-0998.13148
dc.description.abstract

Biological surveys based on visual identification of the biota are challenging, expensive and time consuming, yet crucial for effective biomonitoring. DNA metabarcoding is a rapidly developing technology that can also facilitate biological surveys. This method involves the use of next generation sequencing technology to determine the community composition of a sample. However, it is uncertain as to what biological substrate should be the primary focus of metabarcoding surveys. This study aims to test multiple sample substrates (soil, scat, plant material and bulk arthropods) to determine what organisms can be detected from each and where they overlap. Samples (n = 200) were collected in the Pilbara (hot desert climate) and Swan Coastal Plain (hot Mediterranean climate) regions of Western Australia. Soil samples yielded little plant or animal DNA, especially in the Pilbara, probably due to conditions not conducive to long-term preservation. In contrast, scat samples contained the highest overall diversity with 131 plant, vertebrate and invertebrate families detected. Invertebrate and plant sequences were detected in the plant (86 families), pitfall (127 families) and vane trap (126 families) samples. In total, 278 families were recovered from the survey, 217 in the Swan Coastal Plain and 156 in the Pilbara. Aside from soil, 22%–43% of the families detected were unique to the particular substrate, and community composition varied significantly between substrates. These results demonstrate the importance of selecting appropriate metabarcoding substrates when undertaking terrestrial surveys. If the aim is to broadly capture all biota then multiple substrates will be required.

dc.languageEnglish
dc.publisherWILEY
dc.relation.sponsoredbyhttp://purl.org/au-research/grants/arc/IC150100041
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectEcology
dc.subjectEvolutionary Biology
dc.subjectEnvironmental Sciences & Ecology
dc.subjectbiodiversity
dc.subjectbiological audit
dc.subjectDNA barcoding
dc.subjectmetagenomics
dc.subjectterrestrial
dc.subjectSOIL
dc.subjectCOMMUNITIES
dc.subjectIDENTIFICATION
dc.subjectARTHROPODS
dc.subjectRESOURCES
dc.subjectDIVERSITY
dc.subjectEFFICIENT
dc.subjectPLANT
dc.subjectDIET
dc.titleTesting multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding
dc.typeJournal Article
dcterms.source.volume20
dcterms.source.number3
dcterms.source.startPage732
dcterms.source.endPage745
dcterms.source.issn1755-098X
dcterms.source.titleMolecular Ecology Resources
dc.date.updated2022-01-24T07:29:16Z
curtin.note

This is the peer reviewed version of the following article: van der Heyde, M, Bunce, M, Wardell-Johnson, G, Fernandes, K, White, NE, Nevill, P. Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding. Mol Ecol Resour. 2020; 20: 732– 745, which has been published in final form at https://doi.org/10.1111/1755-0998.13148. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

curtin.departmentSchool of Molecular and Life Sciences (MLS)
curtin.accessStatusOpen access
curtin.facultyFaculty of Science and Engineering
curtin.contributor.orcidBunce, Michael [0000-0002-0302-4206]
curtin.contributor.orcidWhite, Nicole [0000-0002-0068-6693]
curtin.contributor.orcidNevill, Paul [0000-0001-8238-0534]
curtin.contributor.orcidVan Der Heyde, Mieke [0000-0002-1658-9927]
curtin.contributor.orcidFernandes, Kristen [0000-0003-3388-5354]
curtin.contributor.researcheridWardell-Johnson, Grant [I-7097-2013]
dcterms.source.eissn1755-0998
curtin.contributor.scopusauthoridBunce, Michael [55160482300]
curtin.contributor.scopusauthoridWhite, Nicole [37065418600]
curtin.contributor.scopusauthoridNevill, Paul [25630973000] [57218223043]
curtin.contributor.scopusauthoridWardell-Johnson, Grant [6603805322]
curtin.contributor.scopusauthoridVan Der Heyde, Mieke [57191499178]


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