Antidiabetic effects and mechanisms of action of γ-conglutin from lupin seeds
MetadataShow full item record
The glucose modulating properties of lupin have been attributed to its seed protein γ-conglutin. Here we explored the antidiabetic potential of γ-conglutin purified from lupin seeds in-vitro. To mimic the effects of an orally administered supplement, purified γ-conglutin was hydrolysed by gastrointestinal proteolytic enzymes and the resulting peptides evaluated for their antidiabetic effects in pancreatic β-cells and primary human skeletal muscle myotubes. γ-conglutin peptides did not promote insulin secretion in β-cells but elicited a potent insulin-mimetic action by activating insulin signalling pathways responsible for glycogen, protein synthesis, and glucose transport into myotubes. Additionally, the peptides potently suppressed the activity of DPP4 indicating their potential to increase the half-life of incretin hormones in circulation. These results substantiate the health benefits of consuming lupin seeds as part of a healthy diet and can drive the current market for lupins from primarily stockfeed, towards value-added lupin-based food products for human consumption.
Showing items related by title, author, creator and subject.
Tapadia, Mrunmai (2019)Lupin seed proteins have been reported to exhibit hypoglycaemic action in animals and humans. This research focuses on exploring in-vitro anti-diabetic action of proteins extracted from Lupinus angustifolius seed. A novel ...
Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approachesFoley, R.; Jimenez-Lopez, J.; Kamphuis, L.; Hane, James; Melser, S.; Singh, K. (2015)Background - The major proteins in lupin seeds are conglutins that have primary roles in supplying carbon, sulphur and nitrogen and energy for the germinating seedling. They fall into four families; α, β, γ and δ. Interest ...
Narrow-leafed lupin (Lupinus angustifolius L.) ß-conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory-related disease ameliorationLima-Cabello, E.; Alche, V.; Foley, R.; Andrikopoulos, S.; Morahan, G.; Singh, Karam; Alche, J.; Jimenez-Lopez, J. (2017)© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Scope: We have investigated the potential use of ß-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment. Methods and ...