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    Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici.

    Access Status
    Open access via publisher
    Authors
    McDonald, M.
    Williams, A.
    Milgate, A.
    Pattemore, J.
    Solomon, P.
    Hane, James
    Date
    2015
    Type
    Journal Article
    
    Metadata
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    Citation
    McDonald, M. and Williams, A. and Milgate, A. and Pattemore, J. and Solomon, P. and Hane, J. 2015. Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. Fungal Genetics and Biology. 79: pp. 71-75.
    Source Title
    Fungal Genetics and Biology
    DOI
    10.1016/j.fgb.2015.04.012
    ISSN
    1087-1845
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/9842
    Collection
    • Curtin Research Publications
    Abstract

    The wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessory chromosomes. The IPO323 reference, when combined with low-cost next-generation sequencing and bioinformatics, can be used as a powerful tool to assess the presence or absence of accessory chromosomes. We present an outline of a range of bioinformatics techniques that can be applied to the analysis of presence–absence variation among accessory chromosomes across 13 novel isolates of Z. tritici.

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