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    MAP2 provides reliable early assessment of neural injury in the newborn piglet model of birth asphyxia

    Access Status
    Fulltext not available
    Authors
    Lingwood, B.
    Healy, Genevieve
    Sullivan, S.
    Pow, D.
    Colditz, P.
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Lingwood, B. and Healy, G. and Sullivan, S. and Pow, D. and Colditz, P. 2008. MAP2 provides reliable early assessment of neural injury in the newborn piglet model of birth asphyxia. Journal of Neuroscience Methods. 171 (1): pp. 140-146.
    Source Title
    Journal of Neuroscience Methods
    DOI
    10.1016/j.jneumeth.2008.02.011
    ISSN
    0165-0270
    School
    School of Physiotherapy and Exercise Science
    URI
    http://hdl.handle.net/20.500.11937/18964
    Collection
    • Curtin Research Publications
    Abstract

    Reduction in microtubule-associated-protein-2 (MAP2) immunoreactivity is a sensitive and quantifiable early marker of neural injury in rats. This study assessed the reliability of MAP2 as an early marker of neural injury following hypoxia/ischaemia in neonatal piglets, and compared the effects of perfusion and immersion fixation on MAP2 immunoreactivity. Hypoxia was induced in newborn piglets (n = 23) by reducing the FiO2 to 4% for 0, 25, 35 or 50 min. Six hours after the end of hypoxia piglets were killed, and the brain removed and immunolabelled for MAP2. Significant reductions in MAP2 immunoreactivity were seen in cortex, hippocampus, basal ganglia and thalamus. Reductions correlated with duration of hypoxia, pH at the end of hypoxia, cerebral function monitor amplitude and cerebral impedance 6 h after hypoxia, and with early histological evidence of ischaemic changes. Regions with reduced immunoreactivity correlated with areas where damage is present in later histological examination in this model. Immersion fixation with postmortem delays up to 30 min did not affect MAP2 immunoreactivity compared to perfusion-fixed tissue. Results indicate that MAP2 immunoreactivity 6 h after hypoxia/ischaemia is a reliable marker of neural injury in the neonatal piglet. © 2008 Elsevier B.V. All rights reserved.

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