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    Analysis, regulation, and roles of endosomal phosphoinositides

    Access Status
    Fulltext not available
    Authors
    Maffucci, T.
    Falasca, Marco
    Date
    2014
    Type
    Book Chapter
    
    Metadata
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    Citation
    Maffucci, T. and Falasca, M. 2014. Analysis, regulation, and roles of endosomal phosphoinositides, in Conn, P. (ed), Methods in Enzymology: Endosome Signaling Part B, pp. 75-91. Amsterdam: Elsevier.
    Source Title
    Methods in Enzymology
    DOI
    10.1016/B978-0-12-397925-4.00005-5
    ISBN
    9780123979254
    ISSN
    0076-6879
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/22458
    Collection
    • Curtin Research Publications
    Abstract

    Phosphoinositides (PIs) are minor lipid components of cellular membranes that play critical roles in membrane dynamics, trafficking, and cellular signaling. Among the seven naturally occurring PIs, the monophosphate phosphatidylinositol 3-phosphate (PtdIns3P) and the bisphosphate phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2] have been mainly associated with endosomes and endosomal functions. Metabolic labeling and HPLC analysis revealed that a bulk of PtdIns3P is constitutively present in cells, making it the only detectable product of the enzymes phosphoinositide 3-kinases in unstimulated, normal cells. The use of specific tagged-PtdIns3P-binding domains later demonstrated that this constitutive PtdIns3P accumulates in endosomes where it critically regulates trafficking and membrane dynamics.

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