Analysis, regulation, and roles of endosomal phosphoinositides
| dc.contributor.author | Maffucci, T. | |
| dc.contributor.author | Falasca, Marco | |
| dc.date.accessioned | 2017-01-30T12:31:31Z | |
| dc.date.available | 2017-01-30T12:31:31Z | |
| dc.date.created | 2015-10-29T04:09:56Z | |
| dc.date.issued | 2014 | |
| dc.identifier.citation | Maffucci, T. and Falasca, M. 2014. Analysis, regulation, and roles of endosomal phosphoinositides, in Conn, P. (ed), Methods in Enzymology: Endosome Signaling Part B, pp. 75-91. Amsterdam: Elsevier. | |
| dc.identifier.uri | http://hdl.handle.net/20.500.11937/22458 | |
| dc.identifier.doi | 10.1016/B978-0-12-397925-4.00005-5 | |
| dc.description.abstract |
Phosphoinositides (PIs) are minor lipid components of cellular membranes that play critical roles in membrane dynamics, trafficking, and cellular signaling. Among the seven naturally occurring PIs, the monophosphate phosphatidylinositol 3-phosphate (PtdIns3P) and the bisphosphate phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2] have been mainly associated with endosomes and endosomal functions. Metabolic labeling and HPLC analysis revealed that a bulk of PtdIns3P is constitutively present in cells, making it the only detectable product of the enzymes phosphoinositide 3-kinases in unstimulated, normal cells. The use of specific tagged-PtdIns3P-binding domains later demonstrated that this constitutive PtdIns3P accumulates in endosomes where it critically regulates trafficking and membrane dynamics. | |
| dc.publisher | Academic Press Inc. | |
| dc.title | Analysis, regulation, and roles of endosomal phosphoinositides | |
| dc.type | Book Chapter | |
| dcterms.source.volume | 535 | |
| dcterms.source.startPage | 75 | |
| dcterms.source.endPage | 91 | |
| dcterms.source.issn | 0076-6879 | |
| dcterms.source.title | Methods in Enzymology | |
| dcterms.source.isbn | 9780123979254 | |
| curtin.department | School of Biomedical Sciences | |
| curtin.accessStatus | Fulltext not available |