Immunosenescent CD57 +CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART
MetadataShow full item record
HIV-infected individuals responding to antiretroviral therapy (ART) after severe CD4+ T-cell depletion may retain low responses to recall antigens [eg: cytomegalovirus (CMV)] and altered expression of T-cell co-stimulatory molecules consistent with immunosenescence. We investigated the capacity of phenotypically senescent cells to generate cytokines in HIV patients receiving long-term ART (n = 18) and in healthy controls (n = 10). Memory T-cells were assessed by interferon (IFN)-γ ELISpot assay and flow cytometrically via IFN-γ or IL-2. Proportions of CD57brightCD28null CD4+ T-cells correlated with IFN-γ responses to CMV (p = 0.009) and anti-CD3 (p = 0.002) in HIV patients only. Proportions of CD57brightCD28null CD8+ T-cells and CD8+ T-cell IFN-γ responses to CMV peptides correlated in controls but not HIV patients. IL-2 was predominantly produced by CD28+T-cells from all donors, whereas IFN-γ was mostly produced by CD57+ T-cells. The findings provide evidence of an accumulation of immunosenescent T-cells able to make IFN-γ. This may influence the pathogenesis of secondary viral infections in HIV patients receiving ART.
This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/3.0/
Showing items related by title, author, creator and subject.
Tan, D.; Ong, N.; Zimmermann, M.; Price, Patricia; Moodley, Y. (2016)Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are characterized by increased pulmonary and systemic inflammation and commonly caused by bacterial and/or viral infection. Little is known about the ...
CD31 (PECAM-1) is a marker of recent thymic emigrants among CD4+ T-cells, but not CD8+ T-cells or ? d T-cells, in HIV patients responding to ARTTanaskovic, S.; Fernandez, S.; Price, Patricia; Lee, S.; French, M. (2010)Some severely immunodeficient HIV patients experience poor recovery of CD4 T-cell counts on antiretroviral therapy (ART). Evaluation of the function of thymopoiesis in T-cell production in individual patients requires a ...
Detection of HCV-specific IFN-gamma responses in HCV antibody and HCV RNA negative injecting drug usersFlynn, J.; Sacks Davis, R.; Higgs, Peter; Aitken, C.; Moneer, S.; Suppiah, V.; Tracy, L.; Ffrench, R.; Bowden, S.; Drummer, H.; George, J.; Bharadwaj, M.; Hellard, M. (2014)Background: Detectable HCV-specific cellular immune responses in HCV antibody and RNA negative people who inject drugs (PWID) raise the question of whether some are resistant to HCV infection. Immune responses from people ...