Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
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Authors
Hartnell, David
Schwehr, Bradley J
Gillespie-Jones, Kate
Alwis, Dasuni
Rajan, Ramesh
Hou, Huishu
Sylvain, Nicole J
Pushie, Michael J
Kelly, Michael E
Massi, Max
Hackett, Mark
Date
2020Type
Journal Article
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Hartnell, D. and Schwehr, B.J. and Gillespie-Jones, K. and Alwis, D. and Rajan, R. and Hou, H. and Sylvain, N.J. et al. 2020. Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes. Analyst. 145: pp. 3809-3813.
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Analyst
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Faculty
Faculty of Science and Engineering
School
School of Molecular and Life Sciences (MLS)
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Abstract
The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation.